Sequential Design for Microarray Experiments

Sequential Design for Microarray Experiments

A critical aspect in the design of microarray studies is the determination of the sample size necessary to declare genes differentially expressed across different experimental conditions. In this paper, we propose a sequential approach where the decision to stop the experiment depends on the accumulated microarray data. The study could stop whenever sufficient data have been accumulated to iden...

Microarray experiments: considerations for experimental design

Introduction Initially conceived and launched ten years ago, microarray technology has become an attractive choice for researchers to screen the expression of thousands of genes simultaneously. During its short history, the technology has made invaluable contributions to various scientific fields. One example is evident in human cancer research and the development of a prognostic tool based on ...

Three-phase Sequential Design for Sensitivity Experiments

In some experimental investigations each experimental unit has a critical stimulus level that cannot be observed directly. The distribution of these critical levels (or thresholds) over test specimens is of primary interest. In pyrotechnics applications the stimulus (or stress) level, denoted by x, may be the drop height of an explosive, or the pressure on a pile of ammunition, and the response...

Experimental design of DNA microarray experiments.

Design and Analysis of Comparative Microarray Experiments

This chapter discusses the design and analysis of relatively simple comparative experiments involving microarrays. Some of the discussion applies to all the most widely used kinds of microarrays, that is, radiolabelled cDNA arrays on nylon membranes, two-color, fluorescently labeled cDNA or long oligonucleotide arrays on glass slides, or single color, fluorescently labeled, high-density short o...